Coding

Part:BBa_K3722002

Designed by: Gao Zhan   Group: iGEM21_NWU-CHINA-A   (2021-09-29)


MaFMO

Monooxygenase hydroxylates the C3 position of the indole ring and forms 6-Br-indoxyl.


Biological function

Monooxygenase from Methylophaga aminisulfidivorans (MaFMO). And flavin is used as a cofactor when MaFMO hydroxylates the C3 position of the indole ring and forms indigo. Indigo has a maximum absorption peak at 606nm.

Instruction

The coding sequence of target gene was inserted into an expression vectors with BBa_K3722000 to obtain BBa_K3722011. We transformed the constructed plasmid into E. coli BL21 (DE3) to verify its successful heterologous expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None